5/30/2023 0 Comments Knockout font family zipThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. Lane 2: DAPK3 knockout HEK-293T cell lysate (20µg) Lane 1: Wild-type HEK-293T cell lysate (20µg) #Knockout font family zip free full#įor full details of the licenses and patents please refer to our limited use license and patent pages. Red - loading control, ab8245 observed at 37 kDa.Īb51602 Anti-ZIP Kinase antibody was shown to specifically react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51602 and Anti-GAPDH antibody - Loading Control ( ab8245) were incubated overnight at 4 ☌ at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye ® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye ® 680RD) preadsorbed ( ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. Lanes 1- 2: Merged signal (red and green).
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